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KMID : 1189119980020020079
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대한의학유전학회지
1998 Volume.2 No. 2 p.79 ~ p.81
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Analysis of haplotype and coamplification PCR of dystrophin gene and Y-specific gene using PEP-PCR in single fetal cells
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Cheong Kyung-Soon
Lee Dong-Sook
Cho Soo-Kyung
Kim Gi-Chul
Min Eung-Gi
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Abstract
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Genetic amniocenteses were performed in a series of 127 patients as a routine study. Samples from the patients were cultured by in situ method, flask method or both according to the state of amniotic fluid. The overall success rate of culture was 97.6% and no culture failure was observed in the flask method. It took 5 days first of all and 8.15 days average from set-up to harvest and there were 7.2 colonies per dish in in situ method. Therefore, it is suggested that in situ method which decreased the mean culture days and made clonal analyses possible, is a clinically available and even more reliable method in parallel with flask method in prenatal diagnosis.
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KEYWORD
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Amniocentesis, In situ method, Prenatal diagnosis
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